Single-Molecule Localization Microscopy Approach Has Higher Precision

Although various image-based central position estimation (centroid fitting) methods such as 2D Gaussian fitting methods have been commonly used in single-molecule localization microscopy (SMLM) to precisely determine the location of each fluorophore, it is still a challenge to improve the single-molecule lateral localization precision to molecular scale (<2 nm) for high-throughput nanostructure imaging.

Recognizing this, researchers at the Institute of Biophysics of the Chinese Academy of Sciences (Beijing, China) developed a new interferometric single-molecule localization microscopy with fast modulated structured illumination, called repetitive optical selective exposure (ROSE).

ROSE uses six different direction and phase interference fringes to excite the fluorescent molecules. The intensity of the fluorescent molecules is closely related to the phase of the interference fringes.

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