Scanning electron microscopy (SEM) is a powerful tool for imaging biological samples at high resolution. However, using the right fixation methods is important to preserve the sample’s structure and prevent artifacts. Researchers discuss a new method for fixing blood samples for SEM analysis using the May-Grünwald solution. This method is less toxic than traditional methods and provides better preservation of blood cells. Researchers also present a new method for analyzing SEM images to measure the degree of blood cell adhesion to medical devices.
The interaction of blood with medical devices is a critical factor in biocompatibility. When blood comes into contact with a device, proteins, and cells can adhere to its surface. This can lead to several problems, such as inflammation, thrombosis, and infection. SEM is a valuable tool for studying blood-device interactions. It allows to image blood cells and other biological structures at high resolution. However, using the right fixation methods is important to preserve the sample’s structure and prevent artifacts.
Traditional fixation methods for Scanning electron microscopy (SEM) often involve harsh chemicals, such as glutaraldehyde and formaldehyde. These chemicals can damage blood cells, making it difficult to assess their morphology accurately.
Researchers found that the May-Grünwald solution effectively preserves the morphology of blood cells and allows for accurate assessment of their adhesion to medical devices. They have also developed a new method for analyzing SEM images to measure the degree of blood cell adhesion. The new method is based on image segmentation and machine learning. The new fixation and analysis methods provide valuable tools for studying blood-device interactions. These methods can be used to develop new and improved medical devices that are more biocompatible.
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