DEEPscope: A New Era Of Deep-Tissue Imaging

November 8th, 2024 | Applications

Researchers have introduced a revolutionary imaging technology, DEEPscope, that promises to reshape our understanding of the brain. This innovative microscope overcomes the limitations of traditional multiphoton microscopy, enabling deep and wide-field visualization of neural activity at unprecedented resolution.
Multiphoton microscopy, a gold standard for deep-tissue imaging, faces significant hurdles. As imaging depth increases, the field of view shrinks exponentially, making it difficult to observe large-scale neural networks. Additionally, the risk of thermal damage to the tissue limits the achievable depth.
DEEPscope addresses these challenges by integrating several innovative techniques:
Adaptive Excitation System: This system optimizes excitation, ensuring efficient fluorescence generation for large field-of-view imaging.
Multi-Focus Polygon Scanning: This technique allows for rapid sample scanning, enabling high-speed imaging.
By combining these techniques, DEEPscope can achieve high-resolution imaging across a 3.23 x 3.23 mm² field of view, capturing neuronal activity in deep cortical layers of mouse brains.
The potential applications of DEEPscope are vast:
DEEPscope has enabled whole-brain imaging of adult zebrafish, capturing structural details at depths greater than 1 mm and across a field wider than 3 mm. Researchers can now visualize complex neural circuits in living animals at a large scale and depth, providing insights into brain function. DEEPscope can be used to study neurological disorders and develop new treatments.
DEEPscope represents a significant leap forward in neuroscience imaging. By overcoming the limitations of traditional techniques, it offers a powerful tool for exploring the intricate workings of the brain. The techniques employed in DEEPscope can be readily integrated into existing multiphoton microscopes, making it accessible to a wide range of researchers. As this technology continues to evolve, we can expect to gain deeper insights into the brain and its role in health and disease.

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