Brillouin Microscopy For Diagnostic Medicine

Diagnostic imaging significantly improves clinical analysis and medical intervention by providing doctors and scientists with essential visual representations of internal body structures. Researchers are constantly making strides in knowing how different imaging technologies can improve human health. Brillouin microscopy, in contrast to more traditional imaging techniques like confocal fluorescence microscopy, can obtain mechanical data (such as stiffness and viscosity) of biological samples in a non-contact and label-free way.

Dual line-scanning Brillouin microscopy (dLSBM) is being used to improve acquisition speed and lower radiation doses, which are the two major barriers preventing the widespread adoption of this method in biomedicine. While reducing the amount of light irradiation by 80 times for 2D and 3D mechanical mapping, the team recorded speeds of 50 to 100 times quicker than its counterpart using dLSBM.

With this invention, scientists can quickly obtain one mechanical image of a cell cluster. This improved acquisition speed is important because it enables us to investigate details of cell behaviors in almost real-time.

An optical imaging technique called Brillouin microscopy is based on Brillouin light dispersion. (BLS). BLS happens when light reacts with a substance and scatters due to thermal changes or molecular vibrations. Several variables, such as heat, compression, water content, or substance stiffness, can impact vibrations. The latter qualities are the most important for using Brillouin microscopy as a diagnostic instrument.

Read more

Related Content: Achieving Low Photobleaching Using LSFM